2. Partition chromatography applies the principle of countercurrent distribution to columns and involves the use of two immiscible solvent systems: one solvent system, the stationary phase, is supported on a suitable medium in a column and the mixture introduced in this system at the top of the column; the components are eluted by the other system, the mobile phase.
1. ... glass column 2,50 m long and 3,2 mm in diameter (1,8") packed with a suitable support impregnated with a stationary phase phenyl-methyl-silicon (e.g. OV 17 at 3 %).
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