a device that produces ultrasonic pressure waves. It usually consists of a stainless‐steel rod, of diameter from 1–2 mm to about 2 cm, that is activated by an oscillator to produce ultrasound waves and is inserted into a vessel to sonicate a sample.
Dilute BTV antigen to pre-titrated concentration in PBS, sonicate briefly to disperse aggregated virus (if sonicator is not available, pipette vigorously) and add 50 μl to all wells of the ELISA plate.
