plaque - areas of cell destruction in a bacterial colony grown on a solid medium, or tissue culture monolayer preparation due to infection with a virus
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DE: der Plaquetest [U50438]
FR: test de neutralization sur plaque [U50438]
FI: plakkineutralisaatiokoe [U50438]
Prior to the inoculation of the cells the supernatant is mixed with equal parts of a suitably diluted pool of antisera to the indigenous serotypes of IPN virus and incubated with this for a minimum of one hour at 15°C or a maximum of 18 hours at 4°C. The titre of the antiserum must be at least 1/2000 in a 50% plaque neutralization test.
