outdoor experimental system that are delimited and to some extent enclosed. They offer more realism than microcosms due to their larger size and more natural physical conditions but can still provide replications, control of chemical exposure, and some control of the biotic components.
A chronic toxicity study must be carried out unless it can be justified that continued or repeated exposure of fish is unlikely to occur or unless a suitable microcosm or mesocosm study is available.
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