Värvimislahuse valmistamiseks segatakse etanool ja 1,1,1-trikloroetaan või tetrakloroetaan ning segu kuumutatakse veevannil kuni temperatuurini 60…70 °C. Seejärel lisatakse metüleensinine, segatakse hoolikalt ja jäetakse külmkappi temperatuurile 4 °C 12 kuni 24 tunniks jahtuma, seejärel lisatakse jää-äädikhape.
Enzymatic determination of starch and its degradation products including glucose in food products using high performance liquid chromatography (HPLC) ... 4. Reagents and other materials ... 4.11. Glacial acetic acid, 96 % (v/v) (minimum).
Pure acetic acid, often called glacial acetic acid, is a corrosive, colourless liquid (boiling point 117.9 °C [244.2 °F]; melting point 16.6 °C [61.9 °F]) that is completely miscible with water.
