tehnika, mille abil uuritakse eri isenditest pärit nukleiinhapete sarnasust nende hübridiseerumisastme alusel (hübridisatsioonitest)
a process in which a strand of nucleic acid joins with a complementary strand through base pairing. The technique is used to locate or identify nucleotide sequences and to establish the effective transfer of nucleic material to a new host
The following information on the library from which the insert originated, should be known:
(i) the source and method for obtaining the nucleic acid of interest (cDNA, chromosomal, mitochondrial, etc.);
(ii) the vector in which the library was constructed (e.g. lamda GT 11, pBR 322, etc.) and the site in which the DNA was inserted;
(iii) the method used for identification (colony, hybridization, immuno-blot, etc.);
(iv) the strain used for library construction.
