Gel solution. Prepare a gel solutiuon by mixing additives and ampholytes wth the stock gel solution (see 4.1.1). 9,0 ml stock solution 24 mg -alanine 500 il ampholyte pH 3,5 - 9,5 (1) 250 il ampholyte pH 5-7 (1) 250 il ampholyte pH 6-8 (1). Mix the gel solution and de-gas for two to three minutes in an ultrasonic bath or in vacuo. Note: Prepare the gel solution immediately prior to pouring it (see 6.2).
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