Suitable preparations of concentrated virus may be made by simple high speed centrifugation of infectious allantoic fluid and disruption of virus to release the internal nucleocapsid and matrix antigens by treatment with the detergent sodium lauroyl sarcosinate. Acid precipitation may also be used by adding 1N HCl to infective allantoic fluid to give a final pH of 3,5-4,0, chilling for at least one hour at 0°C and low speed centrifugation at 1000 g for 10 minutes.
Good to know
precipitation - the formation of a visible aggregate when antigen and antibody are mixed in aqueous solution so as to form large macromolecular complexes
