Kemoluminestsents erineb tavalisest fluorestsentsist või fosforfluerostsentsist selle poolest, et elektrooniline ergastatud olek on pigem keemilise reaktsiooni kui footoni imendumise tulemus ja valgus tekib keemiliselt eksotermilisest reaktsioonist.
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Ensüüme kasutatakse sageli kemoluminestsentsmeetodites, sealhulgas peroksidaas ja fosfataas ensüümiga seotud immunosorbentanalüüsis.
Chemiluminescence differs from fluorescence or phosphorescence in that the electronic excited state is the product of a chemical reaction rather than of the absorption of a photon. Here, light is generated from a chemically exothermic reaction.
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Enzymes are often used in chemiluminescence methods, including peroxidase and phosphatase in enzyme-linked immunosorbent assay.
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