et

blottimine

1

Terminological databases

EKI terminibaas Esterm

ID 728831 Last modified 20.11.2024
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Domain cell biology
immunoblot preferred
blottimine
immuno-blot preferred
  • geelelektrofreesil lahutatud sarnaste makromolekulide identifitseerimiseks kasutatav tehnika, mis seisneb komponentide ülekandes geelist spetsiaalsele membraanile ja nende järgnevas detekteerimises
  • technique used to detect the presence of a polypeptide which is antigenic to the specific antibody used in the assay. A protein mixture is denaturated and separated on the basis of size by SDS gel electrophoresis, then blotted on to a sheet of nitrocellulose, preserving the pattern due to size. The proteins in the pattern are reacted with the specific antibody, then with an AP- or HRP-conjugated second antibody against the first which after the development, produces visible staining at the site of binding.
immunoblot preferred
blottimine
immuno-blot preferred
Usage examples
  • The following information on the library from which the insert originated, should be known: (i) the source and method for obtaining the nucleic acid of interest (cDNA, chromosomal, mitochondrial, etc.); (ii) the vector in which the library was constructed (e.g. lamda GT 11, pBR 322, etc.) and the site in which the DNA was inserted; (iii) the method used for identification (colony, hybridization, immuno-blot, etc.); (iv) the strain used for library construction.
blotting
immuno-blot
Immunmembran

Biokeemiasõnastik

ID 458217 Last modified 09.08.2021
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siire
laigutus

Geneetika terminibaas

ID 1152178 Last modified 22.12.2025
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  • molekulaarbioloogias kasutatav meetod, mille käigus geelelektroforeesiga (agaroos või polüakrüülamiid) eraldatud valgud ja nukleiinhapped teisaldatakse kandjale (nt nitrotselluloos- või nailonfilter) ja seejärel tuvastatakse spetsiifiliste sondide (antikehad või komplementaarsed nukleiinhapped) abil
blottimine
blotting

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