technique used to detect the presence of a polypeptide which is antigenic to the specific antibody used in the assay. A protein mixture is denaturated and separated on the basis of size by SDS gel electrophoresis, then blotted on to a sheet of nitrocellulose, preserving the pattern due to size. The proteins in the pattern are reacted with the specific antibody, then with an AP- or HRP-conjugated second antibody against the first which after the development, produces visible staining at the site of binding., geelelektrofreesil lahutatud sarnaste makromolekulide identifitseerimiseks kasutatav tehnika, mis seisneb komponentide ülekandes geelist spetsiaalsele membraanile ja nende järgnevas detekteerimises