The function and relative position of the following should be known:
(i) structural genes;
(ii) marker genes for selection (antibiotic resistance, heavy metal resistance, phage immunity, genes coding for degradation of xenobiotics, etc.);
(iii) regulatory elements;
(iv) target sites (nic-sites, restriction endonuclease sites, linkers, etc.);
(v) transposable elements (including provirus sequences);
(vi) genes related to transfer and mobilization function (e.g. with respect to conjugation, transduction or chromosomal integration);
(vii) replicon(s).
